两种高分子支架对骨髓间充质干细胞生长的影响

Effect of Two Kinds of High Polymeric Scaffolds on the Growth of Bone Marrow Mesenchymal Stem Cells

目的探讨骨髓间充质干细胞(MSCs)在两种不同组成和形貌的高分子支架内的生长情况。方法在体外,分别种植MSCs于纳米纤维聚左旋丙交酯(PLLA)支架和7通道、16通道的聚乳酸-羟基乙酸(PLGA)支架中,用扫描电镜、荧光显微镜及MTT检测等技术观察其生长情况。在体内,将10只SD大鼠分为两组,损伤对照组和MSCs移植组。暴露大鼠胸10脊髓段做全横断损伤,其中在损伤对照组脊髓损伤处移植PLGA支架,在MSCs移植组脊髓损伤处移植含有MSCs的PLGA支架。术后两组动物观察60d。结果在扫描电镜和荧光显微镜下均可以观察到MSCs在两种高分子支架内生长,其中16通道的PLGA支架内的存活细胞数量多,分布均匀。MTT检测结果显示,体外培养3d后,在16通道的PLGA支架内的MSCs生长活力比其他两种支架的MSCs生长活力要大。在术后60d,移植在脊髓损伤处PLGA支架内的MSCs仍然有存活。结论16通道的PLGA支架可能是比较好的神经组织工程支架。

Objective To explore the effects of two kinds of high polymeric scaffolds on the growth of bone marrow mesenchymal stem cells （MSCs）. Methods MSCs were separately implanted into nanofibrous PLLA scaffold, and 7-channel and 16-channel PLGA scaffolds in vitro. Scanning electron microscope, fluorescence microscope and MTT assay were applied to investigate the information of MSCs＇ growth in the scaffolds. Ten SD rats were divided into two groups： injured control group and MSCs transplanted group. Thoracic 10 spinal cord segment was exposured, and then transected completely. PLGA scaffold were transplanted into the transected site of spinal cord in the injured control group. PLGA scaffold of containing MSCs were transplanted into the injured site in the MSCs transplanted group. 60 days after operation two groups of animals survived. Results The growth of MSCs in three kinds of high polymeric scaffolds can be observed under the scanning electron microscope and fluorescence microscope. Particularly in the 16-channel PLGA scaffold, surviving MSCs were much more and well-distributed. The result of MTT assay indicated that the growth＇s vigor of MSCs in 16-channel PLGA scaffold was more than those in other two kinds of scaffolds at three days after culture. At 60 days after operation, MSCs in the PLGA scaffold transplanted into the injured site of spinal cord were still surviving. Conclusion 16-channel PLGA scaffold may be a favourable scaffold for nervous tissue engineering.