砾间接触氧化反应器中细菌多样性评价

Phylogenetic Diversity Assessment of Bacteria In a Gravel Contact Oxidation Reactor

采用分子生物学手段,通过构建16S rDNA基因文库,对新型剩余污泥减量化处理系统——砾间接触氧化反应器中细菌多样性进行了系统发育分析;通过多样性指数、丰富度指数、均匀度指数及优势度指数,对细菌多样性及优势菌种富集状况进行了系统评价。结果表明:共有80个克隆子,分别代表29个OTU,用于系统细菌分析。其中,石球外膜46个克隆子(17个OTU),石球内泥34个克隆子(12个OTU)。生物膜中细菌多样性及优势菌种富集状况均好于孔隙内泥中的细菌。

The phylogenetic diversities of bacteria in a gravel contact oxidation reactor, a novel equipment for excess sludge decrement, were studied by constructed 16S rDNA library, PCR- based molecular surveys of environmental 16S rDNA genes. Total 80 16S rDNA gene clones were retrieved and evaluated their phylogenetic diversity. 46 clones （17 OTUs） were isolated from the biofilm, and 34 clones （12 OTUs） were from the inner sludge. The measured bacteria diversities were evaluated by diversity indices, richness indices, evenness indices and dominance indices. The evaluation results indicated that both diversity and dominance of the bacteria isolated from biofilm were higher than inner sludge.