摘要
艾滋病自1981年发现以来就成为全球关注的热点问题,目前尚无安全有效的临床治疗方法,所以防治的主要手段仍以预防为主。血清免疫学实验依然是实验室诊断HIV的主要依据。笔者着重从血清学检测、病原检测和免疫学检测等方面,介绍了实验室检测HIV抗体的血清学方法,以及新近发展的HIV P24抗原测定、核酸检测、生物芯片技术和评价免疫功能的CD_4细胞计数方法。本文总结了近年来国内外有关艾滋病的各种检测技术及其研究进展。
Acquired immuno deficiency syndrome (AIDS) has been a issue since it was first reported in 1981. There is no effective safety method to cure AIDS at present.Therefore, preventive defence is the major measure that usually taken to control the spread of AIDS. Currently, Serum immunological experiment is still the major basis methods used for detection of antibody to human immunodefiency virus (HIV). The article followed discusses the serological method for experimental testing of HIV, and the latest progress of the technologies of HIV p24 antigen test, nucleic acid test, biochip and the CD4 cell counting method for evaluating the immune functions. This paper summarises various technologies of detection of AIDS.