HPLC测定龟鹿补肾丸中淫羊藿苷的含量

Determination of Icariin in Guilubushen Pills by HPLC

目的建立RP—HPLC法龟鹿补肾丸中有效成分淫羊藿苷含量的测定方法。方法采用HPLC法，色谱柱为Hypersil ODS（250mm×4．0mm，5μm）柱，流动相为乙腈-水（27：73），检测波长为270nm。结果淫羊藿苷线性范围0．0264～0．528μg/ml，r=0．9995（n=5）；回收率为100．17％（n=5），RSD为1．0％。结论测定方法简便、稳定，能有效地控制该制剂的质量。

Objective To establish a method for the determination of icariin content, the effective component of Guilubushen pills. Methods The isolation was conducted on a Hypersil ODS column （ 250 mm× 4.0 ram, 5 μm）, the mobile phase consisted of acetonitrol-water （27： 73）, the detection wavelength was 270 nm. Results The content of icariin showed good lineafity in the range of 0. 0264 - 0.528 μg/ml, r = 0. 9995 （ n = 5 ） , and the average recovery was 100.17 % （ n = 5） ,RSD was 1.0%. Conclusion The method is simple, stable, and can effectively control the quality of the preparation.