摘要
根据Ⅰ型鸭病毒性肝炎ZJ/06强毒株的测序结果,在其基因组3D基因区设计合成一对可扩增238 bp的特异性引物,成功的建立了检测DHV-Ⅰ的RT-PCR方法,并确定了其特异性与敏感性,对DHV-Ⅰ分离毒株和临床病料进行RT-PCR检测均得到阳性扩增结果,而作为阴性对照的常见4种鸭病病原:鸭瘟病毒、鸭产蛋综合症病毒、鸭副粘病毒、鸭里默氏杆菌均未扩增到任何片断。利用BHK-21细胞、SPF鸡胚对DHV-Ⅰ临床病料进行病毒分离鉴定,结果表明建立的DHV-Ⅰ的RT-PCR检测方法具有快速、特异、敏感的特点。
According to the results of DNA sequencing of duck hepatitis virus （DHV- Ⅰ ） ZJ/06 virulent strain, a pair of specific primers which could amplify 238 bp fragment in gene region of genome 3D were designed and developed, a RT-PCR method detecting DHV-Ⅰ was developed successftdly, and the specific and susceptibility of DHV-Ⅰ were determined. A specific 238 bp fragment could be amplified from virulent strains and clinic samples of DHV- Ⅰ by RT-PCR method, but no bands were amplified from four kinds of normal duck diseases： duck plague virus （DPV）, duck egg drop syndrome virus （DEDSV）, duck paramyxovirus （DPV） and Pdemerella anatipestifer from duck （DRA）. Some clinical samples of DHV- I were isolated and identified using BHK-21 cell and SPF chicken embryo, respectively. The results showed that the method of RT-PCR setup for detecting DHV-Ⅰ had the character of fast, specific and sensitive.
出处
《浙江农业学报》
CSCD
2007年第6期409-412,共4页
Acta Agriculturae Zhejiangensis
