不同激素组合对康乃馨组织培养的影响

Effects of different hormone combinations on tissue culture in Dianthus carophyllus

研究了1-萘乙酸(NAA)、2,4-二氯苯氧乙酸(2,4-D)和6-苄基腺嘌呤(6-BA)等3种激素的质量浓度组合对康乃馨组织培养过程的影响.结果表明,添加相对少量的NAA有助于6-BA诱导出更多更壮的丛生芽,其中以添加质量浓度6-BA 2 mg/L+NAA 0.5 mg/L和6-BA 2 mg/L+NAA 1 mg/L为效果最佳;2,4-D虽为诱导康乃馨愈伤组织产生所必需,但添加相对低质量浓度的6-BA效果更佳,其中2,4-D 1.0 mg/L+6-BA 0.5 mg/L和2,4-D 2.0mg/L+6-BA 0.5 mg/L为康乃馨愈伤组织诱导的最佳激素配比;单独使用6-BA可使康乃馨愈伤组织分化,添加适量NAA效果更好,6-BA 1.0 mg/L+NAA 0.5 mg/L和6-BA 2.0 mg/L+NAA 0.5 mg/L诱导的芽点最多;但6-BA对丛生芽来源的无根苗的生根起抑制作用,活性炭对生根并无帮助,而以NAA 0.5 mg/L的生根效果最佳.

The effects of the combination of three hormones, i.e. 1 naphthlcetic acid （NAA）, 2,4 dichlorophenoxy acetic acid （2,4-D） and 6-benzylaminopurine （6-BA） on tissue culture progress in carnation （Dianthus carophyllus L, ） were studied, The results show that adding relative lower NAA concentration can help 6-BA to induce much more and stronger clumpy buds, while adding 6-BA 2, 0 mg/L＋NAA 0, 5 mg/L and 6-BA 2.0 rag/L＋ NAA 1.0 mg/I, were the best hormone combinations, Although 2,4-D is necessary for inducing carnation callus, adding relative lower 6 BA is better, and 2,4-D 1, 0 mg/L＋6-BA 0.5 mg/L and 2,4-D 2.0mg/L ＋6-BA 0.5 mg/L are the most suitable hormone combination for callus induction of carnation. 6-BA alone can differentiate callus, but adding enough NAA especially can get more shoots, especially adding 6-BA 1.0 mg/L ＋NAA 0.5 mg/L and 6-BA 2.0 mg/L＋NAA 0.5 mg/L. As regards rooting from clumpy buds, only NAA can induce rooting and NAA 0.5 mg/L is the best hormone concentration, whereas 6-BA has negative effect, and active carbon has no effect in this study.