光合细菌培养基配方的优化研究

Study on the optimization of a culture medium for photosynthetic bacteria

光合细菌培养基的组分是由乙酸钠、氯化铵、磷酸二氢钾、硫酸镁、酵母膏等5种原料构成。采用L16(45)正交表,将5种原料作为光合细菌生长有影响的因素,通过试验数理统计的直观和理论分析,对其配方进行优化。试验结果表明,当培养基的配方为乙酸钠3.3 g/L、氯化铵0.6 g/L、磷酸二氢钾0.9 g/L、硫酸镁0.5 g/L、酵母膏1.5 g/L、光照3 000 lx和温度(32±2)℃时,培养72 h即可达到生长峰值,细菌总数可以从起初的1.75×109cfu/mL提高到3.19×109cfu/mL。优化的培养基条件能有效促进光合细菌生长。

The culture medium for photosynthetic bacteria is composed of CH3 COONa, NH4 Cl, MgSO4, KH2PO4 and Peptone. We use L16 （4^5） orthogonal design test to optimize the culture medium,taking above 5 components as influenciing factors to photosynthetic bacteria growth. According to the results： when the culture medium containing CH3COONa 3.3 g/L, NH4Cl 0.6 g/L, KH2PO4 0.9 g/L, MgSO4 0.5 g/L and Peptone 1.5 g/L, after 72 hour growth under illumination 3 000 lx and temperature （32 ± 2）℃, the photosynthetic bacteria growth ean reach peak value. The total number of bacteria increase from initial 1.75 ×10^9 cfu/mL to 3, 19 ×10^9 cfu/mL. The optimized culture medium can effectively improve photosynthetic bacteria growth.