导痰汤对大鼠脑血管内皮细胞细胞间粘附分子-1的影响

Influence of Daotan Decoction on the Expression of ICAM-1 in Cerebrovascular Endothelial Cells of Rat

目的对导痰汤干预脑血管内皮细胞细胞间粘附分子-1(ICAM-1)的表达进行研究。方法通过脑血管内皮细胞培养,采用免疫组织化学方法观察肿瘤坏死因子α(TNF-α)在不同时程(0～24h)对内皮细胞ICAM-1表达的影响,以及导痰汤的干预情况。结果随着TNF-α作用时程增加,内皮细胞ICAM-1表达显著上调,呈时间依赖性,P<0.05。预先使用导痰汤处理内皮细胞后,ICAM-1表达无显著性变化,与模型组比较,P<0.05。结论导痰汤能对不同时程的TNF-α诱导ICAM-1高表达发挥抑制作用。

Objective To study the intervention of Daotan Decoction to the expression of ICAM-1 in cerebrovascular endothelial cells. Methods Cerebrovascular endothelial cells were cultured to observed the influence of ICAM-1 expression, which was induced by TNF-α, by the way of immunocytochemistry methods. Results Compared with control group, the expression of ICAM-1 in model group was time-dependent （P〈0.05）. And compared with model group, Daotan Decoction could significantly restrain ICAM-1 expression （P〈0.05）. Conclusion Daotan Decoction could restrain ICAM-1 expression induced by TNF-α in different phase.