黄连素对人宫颈癌Hela细胞株的体外作用研究

Study on the Effect of Berberine in Human Cervical Cancer Hela Cells in Vitro

目的研究黄连素对人宫颈癌Hela细胞株的体外作用,探讨其可能的作用机制。方法采用MTT法测定细胞生长抑制率;原位末端标记技术法(TUNEL)检测细胞凋亡率;免疫细胞化学法检测药物作用前后细胞(COX-2)、Bcl-2蛋白水平表达的变化。结果黄连素在体外能以时间和剂量依赖的方式对宫颈癌Hela细胞产生细胞毒作用并诱导其凋亡;免疫细胞化学法证实黄连素作用Hela细胞后Bcl-2蛋白表达下调,COX-2的表达无明显变化。结论黄连素在体外对宫颈癌Hela细胞具有生长抑制作用,并可诱导细胞凋亡,其诱导凋亡作用可能与Bcl-2蛋白表达下调有关。

Objective To investigate the effects and the possible mechanisms of berberine in human cervical cancer Hela cell line in vitro. Methods Cell growth rate was determined with MTT assay. TUNEL was used to examine cell apoptos rate. The expression of COX-2 and Bcl-2 proteins were detected by immunocytochemistry method. Results Berberine induced cytotoxicity and apoptosis in human cervical carcinoma Hela cells in time-dependent and dose-dependent manner. Berberine could down-regulated the Bcl-2 protein expression of Hela cells, but had no significant influence in the expression of COX-2 protein. Conclusion Berberine can inhibit Hela cell growth of cervical carcinoma and induce apoptosis in vitro. Down-regulation of the Bcl-2 protein expression may be involved in berberine-induced apoptosis of HeLa cells.