泰山四叶参提取物对小鼠抗氧化能力的影响

Study on the Antioxidative Effect of the Extracts of Codonopsis Lanceolata from Mount Taishan in Mice

目的观察泰山四叶参乙醇提取物和水提取物的抗氧化作用。方法将小鼠随机分为6组,分别每日1次等容量灌胃给予5%吐温-80(20mL/kg)、维生素C(0.1g/kg)、四叶参醇提物(10、20g/kg)和四叶参水提物(10、20g/kg),给药14d后杀鼠取血、肝、脑,测定其超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和谷胱甘肽(GSH)、丙二醛(MDA)的含量。结果四叶参水提物和醇提物20g/kg剂量组能显著提高血清GSH水平,提高血清、肝匀浆和脑匀浆SOD、GSH-Px活性,降低MDA含量;四叶参水提物10g/kg剂量组血清和肝匀浆SOD、GSH-Px、GSH水平也见明显升高,MDA含量也显著下降。结论泰山四叶参醇提物和水提物均具有一定的抗氧化作用,水提物的作用较醇提物明显。

Objective To study the antioxidative effect of the ethanol extract （e-EXT） and aqueous extract （a-EXT） of Codonopsis lanceolata from Mount Taishan in mice. Method The mice were divided into six groups at random. After the mice were orally treated with 5% Tween-80 （solvent, 20 mL/kg）, Vitamin C （0.1 g/kg）, e-EXT （10, 20 g/kg） and a-EXT （10, 20 g/kg） once daily for 14 days respectively, the blood, liver and brain were collected for determining SOD, GSH-Px, GSH and MDA levels. Results The SOD, GSH-Px and GSH levels were enhanced significantly, and the MDA levels were reduced markedly in serum, liver and brain homogenate of the mice treated with 20 g/kg e-EXT and a-EXT. In the serum and liver homogenate of 10 g/kg a-EXT group, the levels of SOD, GSH-Px and GSH were increased, the contents of MDA were decreased. Conclusion The ethanol extract and aqueous extract of Codonopsis lanceolata from Mount Taishan could enhance the antioxidative ability of the mice, especially the large-dose aqueous extract.