复方元胡止痛贴中延胡索、白芷乙醇回流提取工艺研究

Optimization of Alcohol Refluxing Extraction Technique of Rhizoma Corydalis and Radix Angelicae Dahuricae in Compound Yuanhu Zhitong Patch

目的优选延胡索、白芷乙醇提取工艺条件。方法以延胡索乙素的提取量为考察指标,用高效液相色谱法测定其含量,采用L9(34)正交试验优选提取工艺。结果优选工艺为:以3倍量75%乙醇提取3次,每次提取1.5h。结论优选的提取工艺合理,转移率高。

Objective To optimize the alcohol extraction technique of the medical herbs such as Rhizoma Corydalis and Radix Angelicae Dahuricae. Methods To optimize the alcohol extraction technique with L9（34） orthogonal test. The content of the tetrahydropalmatine in Rhizoma Corydalis, which was used as evaluating criteria, was determined by HPLC. Results The optimized extraction technique was as follows： the medicinal material was extracted for 3 times with 3 times amount of 75% alcohol, 1.5 hours per time. Conclusions The optimized technique is reasonable and the extraction rate was high.