增殖细胞核抗原、表皮生长因子受体和B淋巴细胞瘤基因在非小细胞肺癌中的表达及临床意义

Expression and significance of proliferating cell nuclear antigen, epidermal growth factor receptor and B cell lymphoma-2 gene in non -sm^ll cell lung cancer

目的研究增殖细胞核抗原（PCNA）、表皮生长因子受体（EGFR）和B淋巴细胞瘤基因（BCL-2）3种基因蛋白与非小细胞肺癌（NSCLC）临床病理特征的关系。方法应用S-P免疫组化法检测44倒手术切除NSCLC标本中3种基因蛋白的表达。结果44例标本中37例（84．09％）PCNA过度表达，30例（68．18％）EGFR过度表达，19例（43．18％）BCL-2过度表达。PCNA、EGFR及BCL-2阳性表达率在NSCLC淋巴结有元转移患者间均有统计学意义（P〈0．01或P〈0．05）。PCNA和EGFR阳性表达率与患者生存时间长短组间有统计学意义（P〈0．01或P〈0．05）。但是3种蛋白阳性表达率均与肿瘤大小、分化程度无相关性（P〉0．05）。结论PCNA、EGFR和BCL-2基因过度表达可能与NSCLC的转移、预后有关。

Objective To explore the relationship between the expression of protein of proliferating cell nuclear antigen （PCNA）, Epidermal Growth Factor Receptor （EGFR）, BCL-2 and pathological characteristics of non-small cell lung cancer （NSCLC）. Methods Protein levels of PCNA, EGFR and BCL-2 were detected in 44 NSCLC samples by immunohistochemistry. Results PCNA was positive in 37 cases （84.09%）, EGFR was 68. 18% and BCL-2 was 43.18% respectively. The expressions of PCNA, EGFR and BCL-2 protein between the group of lymph node metastasis and the group of non - lymph node metastasis were significant different （ P 〈 0. 05 ）. The expressions of PCNA and the EGFR showed significant relation with the survival time （P 〈 0.05）. But expressions of the three proteins were not significantly related with the size and differentiation of the tumor （ P 〈 0. 05 ）. Conclusion Overexpression of PCNA, EGFR and BCL-2 may play an important role in the oncogenesis and development of NSCLC.