微波辅助切割胸腺五肽树脂

Microwave Assisted Cleavage of Thymopentin Peptide Resin

采用美国CEM公司MARS 5型微波反应系统,微波辐射下切割胸腺五肽树脂,以HPLC定量,研究了微波辐射下切割试剂、反应时间和反应温度对胸腺五肽产率的影响,探讨了微波作用的机理,并用质谱、核磁共振测试技术对产物进行了表征。结果表明,微波辐射下以V(TFA)∶V(H2O)∶V(TIS)=95∶2.5∶2.5为切割试剂,50℃下反应5 min,胸腺五肽产率达到最高,为93.2%。与常规方法相比,反应速率和胸腺五肽产率分别提高24倍和21.8%,降低了副反应的发生及多肽的制备成本。

Thymopentin resin was cleaved under microwave irradiation with an American Mas 5 microwave reactor. The effects of cleavage reagents, reaction time and reaction temperature on TP5＇s yield, were studied with HPLC. The mechanism of TPS cleaved from thymopentin resin under microwave irradiation was discussed. The product was characterized by MS and ^1H NMR. The results indicate that TP5＇s yield reached up to 93.2% at 50 ℃ with V（TFA） ： V（ H2O） ： V（TIS） = 95： 2. 5：2.5 as cleavage reagent under microwave irradiation for five minutes. Compared with the results of conventional method, the cleavage reaction rate and yield of TP5 were increased by 24 times and 21.8% , respectively, while the side reaction and the cost of peptide preparation were reduced.