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GAP-43对脊髓全横断损伤后的运动功能的影响 被引量:1

Behavioral Score of GAP-43 in Rat Acute Complete Spinal Cord Injury
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摘要 目的通过制备完全性脊髓损伤成年SD大鼠模型,观察脊髓全横断损伤后运动功能和断端脊髓的病理学改变,探讨生长相关蛋白GPA-43对脊髓损伤后的修复作用,为临床治疗提供实验依据.方法咬除T7~T8棘突及相应椎板,用剪刀将脊髓完全横断,制成脊髓损伤模型.雌性8周龄SD大鼠75只,随机分为三组:GPA-43抗体组,GPA-43抗原组,对照组,每组25只.使用直接注射法将GPA-43抗原和GPA-43多克隆抗体分别注入抗原组和抗体组大鼠脊髓的断端,观察各组大鼠的肢体功能恢复情况.用BBB评分法评价不同的时段运动功能,HE染色病理学检查脊髓组织形态学变化.结果三组在运动功能上相比均具有显著差异,对照组在不同时间运动功能评分最低,抗原组评分最高,脊髓损伤区出现明显的病理改变;抗体组早期恢复出现明显的停滞状态,但随后能很快恢复,说明多克隆抗体对神经组织不产生不可逆改变.结论GAP-43对脊髓损伤的恢复有一定的疗效. Objective To study the behavioral changes of the spinal cord injury model to provide important data for neuroal factor therapy of the impaired spinal cord. Methods In SD rat, aged 8 weeks, ( T7-T8 spinous processes as control), after the spinous process and corresponding lamina of vertebra were removed, the spinal cord was cut by using scissors to prepare the complete transversal abruptly spinal model. The behavioral score of the rat was recorded at different time. The evaluation was made in imageology and pathology. Results The score of SD rat in the experiment group was higher than the control group at different control time. The pathobiology and imageology alterations were very apparent in the damaged spinal area. Conclusion GAP-43 has neuroprotective effect on the recovery of spinal cord injury.
出处 《昆明医学院学报》 2007年第6期39-42,共4页 Journal of Kunming Medical College
基金 云南省教育厅青年教师科研基金项目(13000035) 昆明医学院本科生科研立项基金
关键词 脊髓损伤 GAP-43 运动功能测试 Spinal cord injury GAP-43 Behavioral score
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