在鼻咽癌细胞中EB病毒LMP1调控G1/S检测点重要相关蛋白的转录

EBV LMP Regulating G1/S Checkpoint Significantly-related Protein Transcription in the Tet-on-LMP1 HNE2 Nasopharyngeal Carcinoma Cell Line

目的在Tet-on-LMP1 HNE2鼻咽癌细胞中探讨EB病毒编码的潜伏膜蛋白1(LMP1)调控p16和cyclin D1的转录.方法采用Western blot方法、荧光素酶报道系统和RT-PCR方法检测在DOX诱导下不同时间点p16和cyclin D1的启动子活性和转录.结果LMP1可下调p16启动子活性和mRNA表达;同时上调cyclin D1启动子活性和mRNA表达.结论在Tet-on-HNE2鼻咽癌细胞中EB病毒LMP1在转录水平调控G1/S检测点重要相关蛋白p16和cyclin D1的表达.

Objective To investigate on EB encoding latent membrane protein 1 （LMP1） regulating the transcription of p16 and cycin D1 in the Tet-on-LMP1 HNE2 nasopharyngeal carcinoma cell line. Methods p16 and cyclin D1 promoter activity and transcription with DOX induction were detected by western blot assay, luciferase reporter system and RT-PCR at the different timepoint. Results LMP1 downregulated p16 promoter activity and mRNA expression and upregulated cyclin D1. Conclusion EB LMP1 can regulate G1/S checkpoint significantly-related p16 and cyclin D1 expression at the transcriptional level in the Tet-on-LMP1 HNE2 nasopharyngeal carcinoma cell line.