PP2Ac在人不同类型肺癌组织和肺癌细胞中的表达

The Expression of PP2Ac in Various Kinds of Lung Carcinomatous tissue and Lung Carcinomatous cells

目的研究PP2Ac在人不同类型肺癌组织和肺癌细胞中的表达.方法用免疫组化方法从组织水平上观测临床获取人不同类型肺癌标本组织的PP2Ac蛋白表达;用Western blot分子生物学方法从细胞水平上测定两种人肺癌细胞株(YTLMC-90和GLC-82)的PP2Ac和p-AKT蛋白表达.结果免疫组化结果显示:在人肺鳞癌、腺癌和小细胞肺癌三种肺癌组织中的PP2Ac蛋白表达与肺癌患者的性别、年龄和肿瘤分化程度均无明显相关性(P>0.05);在人肺鳞癌组织和小细胞肺癌组织细胞浆中均未见有PP2Ac蛋白表达,但在其间质有阳性表达;在人肺腺癌组织中PP2Ac蛋白呈阴性表达,而p-AKT蛋白却呈现强阳性表达.Western blot结果表明:在人正常肺组织细胞中PP2Ac蛋白呈正常表达;YTLMC-90细胞中PP2Ac蛋白较人正常肺组织表达稍弱,而在GLC-82细胞中表达明显减弱,甚至缺失;在人正常肺组织细胞中p-AKT蛋白呈低表达,给予GLC-82细胞加入PP2A抑制剂OA(岗田酸),在6 h、12 h、24 h的不同时间点,p-AKT蛋白的表达均较未加OA组明显增强,PP2Ac和p-AKT蛋白表达呈反相关.结论PP2Ac可能参与肺癌的发生,其表达水平可能与肺癌细胞病理类型有关.AKT的活化(p-AKT)可能与肺癌的发生发展密切相关.

Objictive To research the expression of PP2Ac in various kinds of human Lung carcinomatous tissue and two kinds of lung carcinomatous cells （YTLMC-9Oand GLC-82）. Methods Immunohistochemical technique analysed the difference expression of PP2Ac protein in collected paraffin-embedded human lung carcinomatous tissue. Westernhlot technique analysed the expression of PP2Ac and p-AKT protein in two kinds of lung carcinomatous cells, YTLMC-90 and GLC-82. Results Immunohistochemical analysis showed that the expression of PP2Ac in various kinds of Lung carcinomatous tissue wasn＇t related to sex, age, differentiation degree （P 〉 0.05） , that there was negative expression of PP2Ac protein in the cytoplasm of the human lung squamous career and small cell lung cancer while positive expression of PP2Ac protein in intercellular substance, and that there were negtive expression of PP＇2A in human lung adencarcinoma and overexpression of p-AKT protein in human lung adencarcinoma. Western blot technique analysis showed that there was nomal expression of PP2A protein in normal human lung tissue, and lower expression in YTLMC-90 cell than in nomal human lung tissue. While sharp low expression GLC-82 cell. Adding okadaic acid （OA） , an inhibitor of PP2A, the expressiong of p-AKT in GLC-$2 cells was sharply higher than in normal lung tissue at 6 h, 12 h and 24 h, indicating that inverse correlation between PP2A and p-AKT protein expression. Conclusions PP2A is involved in human lung carcinomar, its expression level is related to histological type of human lung carcer. Activition of AKT （p-AKT） is related tightly to human lung carcer.