葡萄糖氧化酶抑制法检测食品中镉、锡、铅的残留

Using the Enzyme-Inhibited Reaction Method to Detect Cd,Sn and Pb ions Residuals in Foods

目前检测重金属使用的国标方法灵敏度高、特异性强，但存在仪器费用高等缺陷。为了建立快速、简便、只要比色即可测定镉、锡、铅的酶抑制法，选择葡萄糖氧化酶（GOD）-辣根过氧化物酶（POD）催化体系，优化了试验条件；利用金属离子对酶活的抑制作用，测定了重金属离子镉、锡、铅对葡萄糖氧化酶活力的影响。所测镉、锡、铅检出限分别为1．3，0．4，1．4μg／mL。研究了检测实际样品中镉、锡、铅的方法，加标回收率分别为92．7％～105．7％；标准差≤1．0％。

The presently used GB method for detecting heavy metals is high sensitivity and selectivity, however, the equipments are expensive. This paper aims at establishing a quick, convenient enzyme inhibition method which needs only vis-spectphotometry to detect Cd, Sn, and Pb. The effects of metal ions on the activity of glucose oxidase（GOD）-peroxidase（POD） was deteded by using the restraining effect. The detection limits of Cd,Sn,and Pb are 1.3μg/mL,0.4μg/mL,and 1.4μg/mL,respectively. The rate of recovery of the metal ions ranged from 92.7% to 105.7%,and RSD%≤1. 0% in the samples.