摘要
目前检测重金属使用的国标方法灵敏度高、特异性强,但存在仪器费用高等缺陷。为了建立快速、简便、只要比色即可测定镉、锡、铅的酶抑制法,选择葡萄糖氧化酶(GOD)-辣根过氧化物酶(POD)催化体系,优化了试验条件;利用金属离子对酶活的抑制作用,测定了重金属离子镉、锡、铅对葡萄糖氧化酶活力的影响。所测镉、锡、铅检出限分别为1.3,0.4,1.4μg/mL。研究了检测实际样品中镉、锡、铅的方法,加标回收率分别为92.7%~105.7%;标准差≤1.0%。
The presently used GB method for detecting heavy metals is high sensitivity and selectivity, however, the equipments are expensive. This paper aims at establishing a quick, convenient enzyme inhibition method which needs only vis-spectphotometry to detect Cd, Sn, and Pb. The effects of metal ions on the activity of glucose oxidase(GOD)-peroxidase(POD) was deteded by using the restraining effect. The detection limits of Cd,Sn,and Pb are 1.3μg/mL,0.4μg/mL,and 1.4μg/mL,respectively. The rate of recovery of the metal ions ranged from 92.7% to 105.7%,and RSD%≤1. 0% in the samples.
出处
《北京农学院学报》
2007年第4期59-62,共4页
Journal of Beijing University of Agriculture
基金
北京市重点建设学科资助项目
北京联合大学师范学院资助项目
北京农学院基础科学基金资助项目