红外线及磁场对兔膝骨关节炎软骨损伤的影响

Infrared radiation and magnetic field therapy ameliorates cartilage damage in rabbits with knee osteoarthritis

目的探讨红外线及磁场联合作用对兔膝关节骨性关节炎软骨损伤的治疗效果。方法采用管型石膏伸直位固定右后肢的方法将24只健康雄性新西兰成年白兔复制出膝骨关节炎模型。6周后将24只兔子全部解除石膏固定,并随机分为4组进行实验处理,即模型组、红外线治疗组(红外组)、磁疗组及红外线和磁场联合治疗组(联合组)。分别于治疗开始后1、2、3周处死白兔,每组每周处死2只,观察软骨的大体改变,并切取其造模肢体股骨内髁及胫骨内侧平台关节面软骨行组织病理学(HE染色、免疫组化及原位杂交等)检测,光镜所见依据Mankin's评分标准评分,并作统计学处理。结果联合组的软骨病变程度(包括软骨面的损伤及软骨细胞的增生、紊乱)有显著减轻,联合治疗比单因子治疗有显著性差异(P<0.05),两个单因子治疗组之间没有显著差异(P>0.1)。结论联合应用红外线和磁场能有效减轻骨关节炎的软骨破坏,并可缩短病程,提高疗效,起到相互协同作用。

Objective To evaluate the effect of infrared radiation and magnetic field therapy on cartilage damage in rabbits with knee osteoarthritis. Methods Knee osteoarthritis was induced in 24 adult New Zealand rabbits by prolonged fixation of the knee joint in extension for 6 weeks. The rabbits were subsequently randomized into control group （without treatment）, infrared therapy group, magnetic field therapy group and the combined infrared and magnetic field therapy group. At the end of the first, second and third weeks of the therapy, respectively, 2 rabbits fIom each group were sacrificed to observe the general changes and histopathology of the condylar cartilage of the femur, and the findings were assessed using Mankin scores. Results Compared with other groups, the rabbits in the combined therapy group showed significantly milder cartilage damage （including injury of the cartilage surface and chondrocyte＇s proliferation and disarrangement） with significantly lower Mankin scores （P〈0.05）. No significant differences were found in the findings between the two groups with exclusive infrared or magnetic field therapy （P〉0.1）. Conclusions Combined infrared and magnetic field therapy can effectively alleviate cartilage destruction, shortens the disease course and enhance the therapeutic effects in rabbits with knee osteoaxthritis.