核黄素在5甲基四氢叶酸光降解过程中的作用(英文)

THE EFFECT OF RIBOFLAVIN ON THE POTODEG RADATION OF 5-METHYLTETRAHYDROFOLATE

目的了解核黄素在5甲基四氢叶酸光降解过程中的作用。方法用溶液吸收光谱仪和荧光光谱仪记录待测液体的吸收光谱,并通过光谱分析来研究叶酸的光降解过程。结果5甲基四氢叶酸的吸收光谱在紫外光和蓝色光的照射下保持不变。加入核黄素后,在250nm处很快出现5甲基四氢叶酸光降解产物——5甲基二氢叶酸所特有的峰值。结论紫外光(UVA)和蓝色光不能使叶酸发生光降解,但加入核黄素后,5甲基四氢叶酸很快光降解为5甲基二氢叶酸。

Objective To explore the effect of riboflavin on the photodegradation of 5 - methyhetrahydrofolate （MTHF）. Methods A UV/VIS spectrophotometer and a luminescence spectrometer were used to record the absorption and fluorescence spectra of the solution of riboflavin and 5 - methyhetrahydrofolate. Results The absorption spectra of 5 - methyhetrahydrofolate remains unchanged when exposure to UVA and blue light. In the presence of riboflavin, an absorption maximum at 250nm which is seen for 5 - methyhetrahydrofolate appears quickly. Conclusion 5 -methyhetrahydrofolate in PBS solution does not degrade under blue light and UVA exposure. In the presence of riboflavin, 5 - methyhetrahydrofolate degrades quickly to 5 - methyldihydrofolate.