泡桐离体开花培养系统的建立

Establishment of in Vitro Flowering Culture System of Paulownia Plants

为了缩短泡桐营养生长周期,给泡桐杂交育种提供便利条件,以3种泡桐实生组织培养苗为材料,研究培养基中植物激素、蔗糖浓度及培养温度对南方泡桐、白花泡桐和兰考泡桐离体开花的影响。结果表明:南方泡桐和白花泡桐在温度15℃、蔗糖浓度50g·L-1、培养基分别为MS+0.9mg·L-1NAA+12mg·L-1ZT和MS+1.2mg·L-1NAA+10mg·L-1ZT条件下,离体开花率最高,分别达90%、95%;兰考泡桐在温度25℃、蔗糖浓度50g·L-1、培养基为MS+1.2mg·L-1NAA+10mg·L-1ZT条件下达到93.3%。

In order to shorten the vegetative growth period and provide fine conditions for hybridization breeding of Paulownia plants, the effect of combinations of phytohormone and sucrose in the MS media and temperature on in vitro flowering of Paulownia australis, P. fortunei and P. elongata was investigated. The results indicated that the highest percentage of P. australis and P. fortunei in vitro flowering might reach 90% and 95% under the condition of 15 ℃ culture temperature, 50 g· L^-1 sucrose and MS ＋ 0.9 mg· L^-1 NAA ＋ 12 mg· L^- 1 ZT or MS ＋ 1.2 mg· L^-1 NAA ＋ 10 mg· L^-1 ZT medium respectively, and that of P. elongata 93.3 % under 25 ℃culture temperature, 50g·L^-1 surcose and MS ＋ 1.2 mg·L^-1 NAA ＋ 10 mg·L^-1 ZT medium.