果实特异性启动子驱动丙型肝炎病毒E2基因植物表达载体的构建

The Construction of a Plant Expression Vector Driven by a Fruit-specific Promoter for the Expression of Hepatitis C Virus E2 Gene

构建了果实特异性启动子驱动的含编码丙型肝炎病毒包膜蛋白E2基因的植物表达载体,为研制有效的转基因植物丙肝疫苗打下基础。以载体pCAMBIA2300为骨架引入pBIl21中的GUS基因,用目的片段E2基因替换其中的GUS基因构建了pCAM-BIA2300G-E2载体,将番茄的果实特异性启动子E8引入pCAMBIA2300G-E2载体中构建了pE8-E2植物表达载体。用限制性内切酶消化重组载体,结果表明重组载体都含有所插入的目的片段。果实特异性启动子驱动的含E2基因的植物表达载体的成功构建,为抗丙型肝炎病毒的口服疫苗研究提供了试验基础。

To construct a plant effective expression vector driven by a fruit-specific promoter for the expression of hepatitis C virus E2 gene, and prepare for the development of an effective against hepatitis C vaccine, the following work was done. A simple and practical middle vector, pCAMBIA230G, was constructed by using pCAMBIA2300 which was inserted with GUS gene from the pbi121. The vector,pCAMBIA2300G-E2, was constructed by replacing GUS gene with HCV E2 gene. The tomato fruit-specific promoter gene E8 was introduced to pCAMBIA2300G-E2 to form pE8-E2. Digestion with restriction enzymes proved that all recombinant vectors had the inserts with expec- ted length of the target fragments. Plant expression vector containing HCV E2 gene driven by fruit-specific promoter was successfully constructed, which may provide a use- ful experiment foundation for further studving edible vaccine against HCV.