香草醛缩氨基均三唑席夫碱对肝癌细胞分化相关酶和蛋白的影响

Effect of vanillin amino-s-triazole Sehiff base on the cell differentiation associated with enzyme and protein from hepatocarcinoma cell

目的：研究香草醛缩氨基均三唑席夫碱对人肝癌细胞SMMC-7721的诱导分化作用。方法：用不同剂量的3-吡啶-3-基-4-[（4-羟基-3-甲氧基-苯亚甲基）氨基]-5-甲硫基-l，2，4-三唑（LH-37）与SMMC-7721细胞共同培养，以MTF法检测细胞增殖和LH-37对细胞增殖的抑制作用；镜下观察细胞形态改变；采用酶联免疫法检测细胞甲胎蛋白含量，金氏法检测细胞碱性磷酸酶活性的变化。结果：LH-37在1×10^-5-1×10^-3mol／L的浓度范围均能抑制细胞增殖且抑制率随浓度的的增加而增高（P〈0．05或P〈0．01），细胞形态和结构向正常肝细胞方向逆转，1×10^-5mol／L和1×10^-6mol／L的LH-37均可使细胞甲胎蛋白含量明显下降，碱性磷酸酶活性明显升高。结论：香草醛缩氨基均三唑席夫碱对人肝癌SMMC-7721细胞具有抗增殖、促分化作用。

Objective： To study the effect of 3 - pyridin - 3 - yl - 4 - [ （ 4 - hydroxy - 3 - methoxy - benzylidene） amino] -5 - methylsulfanyl -4H - [ 1, 2, 4] triazole （ LH - 37） on induction of differentiation of hepatocarcinoma SMMC - 7721 cells. Methods： SMMC -7721 cells were cultured in RPMI - 1640 and treated by LH -37 at different dose. The proliferation of the cells was examined by MTT assay. Cell morphology was observed by a light microscopy. ELI SA method was used to detect the changes of alpha fetoprotein（ α - FP） contents of the cells. The specific activities of alkaline phosphatase（ALP） were assayed by ALP kit based on King＇ S method. Results： The cell proliferation was inhibited by LH -37 at 1 × 10^-5 - 1 × 10^-3mol/L in dose dependent manners. 1 × 10^-5mol/L LH -37 induced the mature and normality of morphology of SMMC-772 1 cells. After treated with 1 × 10^-5mol/L or 1 × 10 ^-6mol/L LH-37, the contents of α- FP was significantly reduced and the activities of ALP were remarkably declined（ P 〈 0.0 1）. Conclusion： LH - 37 may inhibit proliferation and induce differentiation of human hepatocarcinoma SMMC -772 1 cells.