摘要
体外研究了不同质量浓度5、20、40 mg/L棉酚对瘤胃厌氧真菌和瘤胃细菌发酵稻草活力的影响。瘤胃真菌发酵试验中,各处理组的底物消失率均显著低于对照组(0)(P<0.05)。产气量结果表明,20、40 mg/L两试验组的发酵明显滞后于其它各组,且发酵24 h和48 h的累计产气量显著低于对照组及51、0 mg/L试验组(P<0.05),说明20 mg/L和40 mg/L的棉酚可显著抑制瘤胃真菌的发酵。在瘤胃细菌发酵试验中,51、0 mg/L棉酚处理组的底物消失率与对照组(0)无明显差异,而20和40 mg/L棉酚处理组的底物消失率显著低于对照组(P<0.05)。累计产气量结果表明,各浓度水平的棉酚对瘤胃细菌发酵无明显的滞后效应。因此,瘤胃真菌对棉酚较为敏感,而瘤胃细菌对棉酚有较强的耐受能力,但高浓度的棉酚对细菌发酵活力有一定的抑制作用。
This experiment was conducted to study the effect of gossypol on the fermentation activity of rumen anaerobic fungi and rumen bacteria in vitro. Fungal and bacterial inocula was incubated with ground rice straw in a habitat simulating medium containing gossypol (5 mg/L, 10 mg/L, 20 mg/L or 40mg/L). The results showed that, for the anaerobic fungi, the digestibility of rice straw in four treatment are significantly lower than the control (P 〈 0.05), and the lag phase of the treatments of the gossypol concentration at 20 mg/L and 40 mg/L are longer than the control, and cumulative gas production of the treatments of the gossypol concentration at 20 mg/L and 40 mg/L are lower significantly than other treatment and control (P 〈 0.05). In the experiment of fermentation of rumen bacteria, the results showed that there is no significantly change for the treatments at 5 mg/L and 10 mg/L compare to the control in rice straw digestibility. However, the treatments at 20 mg/Land 40 mg/L are significantly lower than the control in digestibility and total gas production (P 〈 0.05). Furthermore, all treatments have no significant lag phase effect on the fermentation of rumen bacteria.
出处
《金陵科技学院学报》
2007年第4期68-71,共4页
Journal of Jinling Institute of Technology
基金
国家自然科学基金(30600461)
江苏省自然科学基金(BK2007536)
集美大学科研基金资助(C60616)
关键词
棉酚
瘤胃真菌
瘤胃细菌
体外发酵
gossypol
rumen fungi
rumen bacteria
fermentation in vitro