三氟拉嗪对癫痫大鼠海马组织NO、SOD及MDA的影响

Effect of Trifluoperazine on the Changes of NO,SOD and MDA in Hippocampus of Epileptic Rats Induced by Pentylenetetrazol

目的观察三氟拉嗪(TFP)对癫痫大鼠脑组织丙二醛(MDA)含量、一氧化氮(NO)含量和超氧化物歧化酶(SOD)活性的影响。方法健康SD大鼠38只,分5组,采用戊四氮腹腔注射制作癫痫模型,测定各组大鼠海马一氧化氮、丙二醛的含量和超氧化物岐化酶的活性。结果与对照组相比,癫痫组、三氟拉嗪3个剂量组大鼠海马一氧化氮及丙二醛水平均增高,且一氧化氮与丙二醛含量呈显著正相关,超氧化物岐化酶活力降低(P<0.05);与三氟拉嗪3个剂量相比,癫痫组大鼠海马一氧化氮及丙二醛水平升高尤为明显,超氧化物岐化酶活力明显降低(P<0.05),一氧化氮含量与超氧化物岐化酶活力呈负相关。结论癫痛发作可以导致的海马组织SOD的大量消耗及NO、MDA的大量生成,三氟拉嗪能降低癫痫大鼠海马组织SOD的消耗及NO、MDA的大量生成,减轻癫痫发作引起的脂质过氧化,对癫痫大鼠海马具有保护作用。

Objective To study the effect of trifluoperazine （TFP） on the concentration of malondialdehyde （MDA） and the enzyme activity of superoxide dismutase （SOD） in the hippocampus of epileptic rats. Methods The models of epilepsy were made by intraperitoneal injection of pentylenetetrazol and the contents of NO, MDA and SOD of all the epileptic hippocampus were assayed and dealt with all data by statistical analysis. Results The latent period of seizure in the three TFP group was obviously prolonged. The contents of NO and MDA in the seizure group and the three TFP group increased compared with the control group （P 〈 0. 05） , and the level of NO and MDA presented significant positive correlation （ P 〈 0. 01 ）. The SOD vitality decreased （ P 〈 0.05）. Compared with the three TFP group, the content of NO and MDA of seizure group increased obviously and the SOD vitality decreased （ P 〈 0. 05）. The level of NO and MDA presented significant negative correlation （ P 〈 0. 01 ）. Conclusions Seizure can result in a great deal of consumption of SOD in hippocampus and quantities of productions of NO and MDA, TFP could scavenge free radicals in the brain tissue of PTZ - induced epileptic animals.