摘要
目的：探讨P120连环蛋白（P120catenin，P120ctn）及E-钙粘蛋白（E-cad-herin。E-cad）在宫颈鳞癌组织和宫颈鳞癌细胞系（Caski、Siha）中的表达、分布及其生物学意义。方法：免疫组织化学、免疫细胞化学及RT—PCR检测正常宫颈组织、宫颈鳞癌组织、人脐静脉内皮细胞（HECV）、Caski、Siha细胞系中P120ctn及E—cad的表达与分布。结果：①正常宫颈组织中P120cm及E—cad表达在胞膜，胞浆少见；宫颈鳞癌中P120ctn及E—cad胞膜表达降低，出现胞浆表达；②HECV细胞系中P120ctn大部分表达在胞膜，E—cad胞膜表达量较少；Caski、Siha细胞系中P120ctn胞膜表达量减少、胞浆表达量增加，E—cad胞膜胞浆表达都较少；③HECV及Caski、Siha细胞系中都有P120ctn与E—cadmRNA表达，P120ctnmRNA在HECV、Caski、Siha细胞系中无表达量差异，E—cadmRNA在Caski、Siha细胞系中表达量低于HECV。结论：P120ctn及E—cad的异常表达与宫颈鳞癌发生发展密切相关，异常表达与肿瘤细胞分化程度、淋巴结转移有关，与年龄、临床分期无关。
Objective： To research P120 - catenin（P120ctn） and E - cadherin （ E - cad ） abnormal expression, distribution and biologic significance in samples of squamous cervical carcinoma（SCC） and SCC cell lines（ Caski ,Siha ）. Methods： Expression and distribution of P120ctn and E - cad were examined in SCC tissue, normal cervical epithelia tissue, HECV, Caski and Siha by immuno- histochemical staining （ SP method） , immuocytochemistry（ICC） and RT - PCR.Results： The results showed that①in the normal cervical epithelia tissue, P120ctn and E - cad were stained in the membrane and low expression in the cytoplasm; In SCC, P120ctn and E - cad found in the cytoplasm, and reduced expression in membranous; ② in the cell lines, P120ctn in HECV were found in the membrane, E - cad expressed little in the membrane ; P120ctn in Caski, Siha was mainly expressed in the cytoplasm, E - cad was found little expression in both of the cytoplasm and membranous; ③mRNA of P120ctn and E -cad were expressed in the cell lines （HECV, Caski and Siha） , P120ctn mRNA was not different between normal and cancer cell lines, E - cad mRNA shows low expression in Caski and Siha compared with HECV. Conclusions： Abnormal expression of P120ctn and E - cad were significantly correlated with generation and development of SCC, also correlated with tumor differentiation status and lymph node metastasis, no significant association with clinical stages and age.
出处
《实用医学进修杂志》
2007年第4期213-218,共6页
Journal of Practical Training of Medicine
