猫传染性腹膜炎的诊断

Diagnosis of Feline Infectious Peritonitis

目的对临床疑似猫传染性腹膜炎病例进行分子生物学、病理学诊断。方法根据冠状病毒3′UTR保守基因序列设计一对引物，对4例病死猫的组织器官和10例腹水进行RT-PCR检测，并对扩增产物进行序列测定；同时对所有病死猫组织器官进行组织病理学诊断。结果RT-PCR扩增产物克隆测序结果与GenBank中猫传染性腹膜炎病毒基因序列同源性为97％～98％。小肠浆膜、肝脏、脾脏等组织可观察到典型的炎性肉芽肿样变、灶性坏死和炎性细胞浸润。结论证实了4只病死猫感染了猫传染性腹膜炎病毒，且10例腹水中检测到猫传染性腹膜炎病毒核酸。

Objective To confirm diagnosis of feline infectious peritonitis by the method of molecular biology and pathology. Methods A pair of primers was designed according to 3′UTR conservative domain of coronavirus genome. A reverse transcriptase PCR （RT-PCR） was carried out in some tissues from 4 cats died because of feline infectious peritonitis and ascitic fluid of 10 cats with feline infectious peritonitis. PCR product was sequenced. Histopathological examinations of the tissues from the died cats were performed. Results 97 ～ 98 percent of the sequence of the PCR product was identity with the sequence of feline infectious ＂peritonitis virus gene in GenBank. The disease was pathologically characterized by disseminated inflammatory granuloma, focal necrosis, and infiltration of mixed inflammatory cells in small bowel serosa,liver, spleen, etc. Conclusion Diagnosis of feline infectious peritonitis has been confirmed in the four cats, and 10 ascitic fluids contained nucleic acid of feline infectious peritonitis virus.