电加热甲醛薰蒸灭菌屏障系统的试验研究

An Experimental Study on Sterilizing Effect of Fumigating of Formaldehyde by Electrically Heating on Barrier System

目的探讨应用电加热甲醛薰蒸灭菌对屏障系统的消毒效果。方法将甲醛溶液置入电热水器内加热薰蒸灭菌,不用高锰酸钾;薰蒸后用血平皿培养基暴露30 min,进行落下菌检测;在37℃恒温箱内培养,一周后观察血平皿内落下菌的个数。结果经过4 h薰蒸,屏障系统内落下菌检测结果为0.42个/血平皿,达到SPF动物饲养环境的要求。结论用电加热甲醛薰蒸灭菌法可以进行屏障系统的消毒处理。

Objective To investigate sterilizing effect of fumigating of formaldehyde by electrically heating on barrier system. Methods Formaldehyde solution was heated in the electric boiler without potassium hypermanganate to fumigate the barrier system. The culture plate was exposed to the barrier system for 30 minutes, then was incubated for a week in 37 ℃ incubator. The fallen bacteria count was carried out. Results The fallen bacterium in the barrier system was 0.42 per plate after being fumigated for 4 hours, which was in keeping with the demands of barrier system. Conclusion The method of fumigating of formaldehyde by electrically heating could be applied to sterilize the barrier system for laboratory animal.