无锡市2005～2006年度流感监测病原分离概况

Summary of flu surveillance in Wuxi City from 2005 to 2006

目的:对无锡地区流感流行情况进行病原学检测,为流感疫情的监控提供依据。方法:从就诊流感样病例中采集鼻咽拭子,用狗肾传代细胞(MDCK)作病毒分离、0.75%的人"O"型红细胞作微量血凝试验(HA),血凝阳性者用亚型血清作微量血凝抑制试验(HI)。结果:2005年10月至2006年12月共做标本1062份,分离出流感病毒254株,平均分离率为23.92%,最高月份为63.41%。分离的病毒型别为甲1型139株,时空分布主要在2005年冬及2006年夏;甲3型1株,在2006年8月份检出;乙型Victoria系114株,主要集中在2006年春季。结论:无锡地区流感流行流行趋势基本为两个峰,冬春季是主峰,夏季有一个次高峰。2006年8月,我们又分离到1株甲3型病毒,提示我们在2006年冬至2007年春可能来临的流感疫情中应予以关注。

Objective： To provide the information of the flu epidemic situation, we detected the flu virus in Wuxi area. Methods：The flu virus in swatches of nasopharynx from clinical flu cases were separated by MDCK cell line, and the he-magglutinin （HA） were performed with the 0. 75% blood type of ＂O＂. The positivity of HA would be analyzed by the hemaggluti- nin inhibition （HI） with the serum of sub-type. Results： The 1062 swatches were analyzed from October 2005 to December 2006, and the 254 flu virus strains were separated, the separation ration was 23.92%, and the high ratio in the month of the time was 63.41%. Separately, 139 strains of H1N1, which distributed from the Winter of 2005 to the Summer of 2006, 1 strain of H3N2, which were detected in August 2006 and 114 strains of Victoria flu virus sub-type B, which collected in the Spring 2006. Conclusion：The flu epidemic situation is that the prevalence of flu emerges in Winter and Spring, and the Summer is the second prevalence of flu. We have separated a strain H3N2 in August 2006, this suggests that we must pay attention to the flu epidemic situation from the Winter 2006 to Spring 2007.