高Fe^(2+)对大豆叶片光合作用的影响

Influence of excessive Fe^(2+) on photosynthesis of soybean leaves

以1601、浙春3号2个大豆(Glycine max(L.)Merrill)品种为材料,研究高Fe2+对大豆叶片光合特性的影响。结果表明,高Fe2+(300 mg/kg和500 mg/kg)抑制大豆叶片光合作用,降低叶片叶绿素含量、净光合速率、蒸腾速率和水分利用率。高Fe2+还破坏叶肉细胞叶绿体结构,内部积累较大淀粉粒及较多脂滴,基粒类囊体肿胀,垛叠松散,排列无序,叶绿体形状畸变,部分被膜破损。可见,高Fe2+胁迫对大豆叶片结构和功能都产生了负面影响。2个大豆品种对高Fe2+的反应存在一定的基因型差异。

A potted experiment was conducted on two soybean （Glycine max L. Merrill） varieties （1601 and Zhechun No. 3） with three treatments. The leaves photosynthetic characteristics and ultrastrueture at full podding period were determined. The results showed that high concentration of Fe^2＋ （300 mg/kg, 500 mg/kg）inhibited photosynthetic activity, leaf chlorophyll content, net photosynthetic, transpiration rate and water utilization efficiency respectively. Ultrastructural observation showed that high concentration of Fe^2＋ resulted in swelling of chloroplast, disruption of chloroplast membrane, some huge starch grains and lipid in the chloroplast,the granum thylakoids piled up loosely and disorder arrangement. Two test soybean varieties had definite genetic difference in response to Fe^2＋ toxicity,and Zhechun No. 3 soybean was more sensitive than 1601.