摘要
在固体培养体系中就麦穗离体时气温、甘露醇预处理及激素ABT各因子对大麦花药愈伤组织诱导及其分化的影响进行了研究。结果表明：预处理前供体所处气温高低对愈伤组织产量有一定的影响；甘露醇预处理效果优于低温处理；ZT和ABT连用对愈伤组织分化具有良好的促进作用，ABT最适浓度为0．3mg／L。
The experiments were carried out for determining the effects of airtemperature on the day of collecting barley spikes,mannitol pretreatment or ABT treatmenton induction of callus or regeneration of green plant in solid-medium culture system. Theresults showed: air temperature on the day of collecting spikes had some effect on callusyield, the effect of mannitol pretreatment was better than that of low-temperaturepretreatment, addition of ZT and ABT to regeneration medium had a good effect for thedifferentiation of callus,the optimum concentration of ABT was 0.3 mg/L.
出处
《上海农业学报》
CSCD
1997年第2期19-22,共4页
Acta Agriculturae Shanghai
关键词
大麦
花药培养
气温
甘露醇预处理
ABT
Anther culture of barley
Air temperature
Mannitol pretreatment
ABT
Callus yield
Green plant yield
Regeneration rate of green plants
