环氧化酶在宫颈癌中的表达及其临床意义

The Expression of Cyclooxygenase-1 and Cyclooxygenase-2 in Cervical Carcinoma

目的:研究环氧化酶(COX)在正常宫颈上皮组织宫颈上皮内瘤变(CIN)及宫颈癌中的表达,探讨其与宫颈癌临床、病理特征的关系。方法:采用组织芯片技术结合免疫组织化学PV法分别检测101例宫颈癌、12例宫颈上皮内瘤变和正常宫颈上皮组织中COX-1、COX-2的表达。结果:①COX-1在正常宫颈组织、CIN及宫颈癌中的表达差异无统计学意义(P>0.05)。COX-2在正常宫颈组织中无表达,在CIN(66.7%)及宫颈癌(87.1%)中的表达率明显高于正常宫颈组织(P<0.01),但COX-2在CIN及宫颈浸润癌中的表达率比较,差异无统计学意义(P>0.05)。②宫颈癌中COX-1的表达与发病年龄、临床分期、病理分级、宫颈浸润深度及淋巴结转移无关(P>0.05);COX-2的表达与发病年龄、临床分期、病理分级及宫颈浸润深度无关(P>0.05),而与淋巴结转移有关(P<0.05),伴淋巴结转移者COX-2的表达较高。结论:COX-1的表达可能与宫颈癌的发生、发展无直接关系;COX-2过度表达可能参与宫颈癌的发生、发展过程,故可作为反映宫颈癌生物学行为的有效指标之一。

Objective： To investigate the expression of COX in cervical carcinoma, CIN and normal cervical epithelium; To evaluate the correlation between the COXs and clinico - pathologic variables in these cervical carcinoma patients. Methods：The expression of COX- 1 and COX - 2, were examined by immunohistochemistry PV method and tissue chip method in 101 cases of cervical carcinomas , 12 cases of CIN and 10 cases of normal cervical epithelium. Results： （DThe positive expression rates of COX - 1 have no significant difference between cervical carcinomas, CIN and normal cervical epithelium. COX - 2 protein can not be detected in normal cervical ep- ithelium; The positive expression rates of COX - 2 in CIN （66.7%） and cervical carcinomas （87.1% ） were significantly higher than in normal cervical epithelium （ P 〈 0.01 ）, but there was no significant difference between CIN and cervical carcinomas（ P 〉 0.05）. OThe expression of COX - 1 in cervical carcinomas has no correlation with age, clinical stages, grades, invasion deep and metastasis of lymphatic node;The expression of COX - 2 in cervical carcinomas has no correlation with age, clinical stages, grades and invasion deep, but it has a correlation with metastasis of lymphatic node （ P 〈 0.05）. Conclusion ： COX - 1 probably has no significant relationship with carcinogenesis and development of cervical cancer; COX - 2 may play an important role in carcinogenesis and development of cervical cancer, COX - 2 may be valuable to further understand the biological behavior of cervical carcinoma.