纤维粘连蛋白的AFM分析

AFM analysis of fibron ection

利用原子力显微镜(AFM)分析了纤维粘连蛋白(FN)单分子及其聚集行为,对FN在壳聚糖膜表面聚集过程及FN/壳聚糖微胶粒形成过程进行了连续观察分析,并分析了微空间中"水膜"在FN分子及微胶粒形成及聚集过程中的作用.FN分子单体呈"V"型,其聚集体以头-尾的方式结合.

The single molecule/dipolymer of Fibronection （FN） and the formation of FN/chitosan protomere were analyzed using atomic force microscope （AFM）, and the role of ＂water film＂ in micro-space of specimen surface during FN and FN/chitosan protomere forming process was also evaluated. The results indicate that FN molecules present ＂V＂ in shape, and two monomers connect by head-to-end to form dipolymer.