RP-HPLC法测定八珍益母片中阿魏酸的含量

Determination of Ferulic Acid Bazhenyimu Tablets by RP-HPLC

目的建立八珍益母片中阿魏酸的含量测定方法。方法反相高效液相色谱的条件为：Agilent—ODS-3（5μm，4．6mm×250mm）柱；流动相：甲醇-0．1％磷酸溶液（30：70），柱温为35℃，检测波长为320nm，流速为1．0mL·min^-1。结果阿魏酸在0．0404-0．404μg范围内呈良好的线性关系，r=0．9999，平均回收率为98．59％，RSD=1．06％。结论本方法快速准确，重现性好，专属性强，为八珍益母片的质量控制提供了依据。

Objective To establish a method for the determination of Ferulic acid in Bazhenyimu tablets.Methods Ferulic acid was determined in Radix Angelicae Sinensis and Rhizoma Chuanxiong of Bazhenyimu tablets by RP-HPLC. Results Ferulic acid showed that have a good linear relationship within a range of 0. 040 4 - 0. 404μg ,r =0. 999 9. The average recovery was 98.59% and RSD was 1.06%. Conclusion This method is specific and accurate with a good repeatability and can be used for the quality control of Bazhenyimu tablets.