摘要
目的建立HPLC法测定丹七片中丹酚酸B的含量。方法色谱柱为AgilentHypemil C18柱，（4．0mm×250mm，5μm）；流动相：甲醇-乙睛-1、5％甲酸溶液（30：10：60）；流速：1mL·min^-1；检测波长286nm；柱温：室温。结果线性范围为11．36～568μg·mL^-1（r=0、99997），平均回收率为101．0％，RSD=1．89％，重复性RSD=0.52％（n=6），精密度RSD=1．20％（n=6），稳定性RSD=1.96％。结论方法稳定、可靠，可作为该制剂的质量控制方法。
Objective To establish a method for determination content of salvia molic acid B in Danqi tablets. Methods Chromatographic conditions was as follows： a Agilent Hypersil C18（4.0 mm × 250 mm,5 μm） column, methanol-acetonitrile-1.5% formic acid （30： 10： 60） as the mobile phase, and UV detector wavelength at 286 nm. Results Alinear ranger of 11.36 - 568 g·mL^-l（r=0.999 9） and mean recovery of 101.0% （RSD = 1.89% ）, precision with RSD of 0.52% （n = 6）, repetition with RSD of 1.20% （n = 6）and stability with RSD of 1.96% were obtained. Conclusion The method is accurate, reliable, and can be used for quality control of the production.
出处
《现代中药研究与实践》
CAS
2007年第6期43-45,共3页
Research and Practice on Chinese Medicines
