多系统酶类结果的偏差评估

The Comparative Study on the Results with Different Biochemical Detecting Systems

目的:时本校三个生化检测系统酶类项目进行方法比对和偏差评估。方法:参考NCCLS的EP9-A文件,以罗氏7600生化分析仪、罗氏原装试剂、cfas校准品和质控品组成的检测系统为比较方法,检测系统1～2为实验方法,用患者新鲜血清对ALT、AST、ALP、GGT进行检测,计算实验方法(Y)和比较方法(X)之间的相对偏盖(SE%).判断不同检测系统的可比性。结果:ALP、GGT在三个检测系统间预期偏差均可接受。ALT、AST仅在两个检测系统间预期偏差可接受。结论:ALP、GGT检测结果在三个检测系统间具有可比性。日立7600生化分析仪和强生250干式生化分析仪AST、ALT的结果不能互认。

Objective To explore the comparability of serum enzyme results of different biochemical detecting systems. Methods According to EP9-A file of NCCLS, three biochemical detecting systems, VITROR250, Roche 7600 and HITACHI 7600, were used to detect ALT,AST, ALP and GGT to obtain the correlation coefficient and the linear equation, respectively. Then the coefficient and equation were used to evaluate the system bias between laboratory methods and comparison method. Results The outcome biases of ALP and GGT could be accepted in three systems, the biases of ALT and AST were not acceptable partly. Conclusion The comparability of ALP and GGT results in 3 detecting systems accorded to evaluation of clinical acceptability. ALT and AST were not compared each other in 2 detecting systems.