p38在榄香烯致鼠胶质瘤C6细胞周期阻滞中的作用

Effect of p38 MAPK on elemene-induced cell cycle arrest in C6 glioblastoma cells

目的探讨莪术提取物——榄香烯对神经胶质瘤细胞的细胞周期的影响及相关机制。方法采用流式细胞分析、免疫印迹等方法分别检测榄香烯对大鼠胶质瘤细胞的细胞周期的影响和p38蛋白表达的变化，并观察p38抑制剂和其显性失活突变体对榄香烯作用的影响。结果经榄香烯40、60及80μg/ml处理后，细胞周期结果显示G0／G1期细胞百分数依次增加11％、16．95％、19．57％，且榄香烯可明显上调磷酸化p38蛋白表达，呈时间、剂量依赖性。SB203580及转染DN—p38能抑制p38的激活，阻断榄香烯的作用。结论榄香烯能诱导胶质瘤细胞的G0／G1期的阻滞，上调磷酸化p38蛋白质表达可能是榄香烯诱导C6细胞周期阻滞的机制。

Objective To observe the effect of elemene on cell cycle of rat C6 glioblastoma cells. Methods Cell cycle analysis and expression of p38 in C6 glioblastoma cells under elemene treatment were measured by flow cytometry and Western blot. Flow cytometry and MTT assay were used to examine cell cycle and cell proliferation while C6 glioblastoma cells were pretreated with p38 inhibiotr and DN-p38 plasmids. Results The fraction of C6 in the G0/G1 phase increased 11%, 6. 95%, 19.57% respectively in the presence of 40, 60 and 80 μg/ml elemene. The level of phosphorylated p38 MAPK was greatly increased in a dose and time-dependent manner. Inhibition of p38 MAPK activation with SB203580 and DN- p38 blocked elemene-induced anti-proliferation effect. Conclusion Elemene could induce G0/G1 cell cycle phase arrest of C6 glioblastoma cells through up-regulation of phosphorylated p38.