尿液水通道蛋白-2与低肾素型高血压关系的研究

Effects of urinary aquaporin-2 in low renin essential hypertension

目的探讨水通道蛋白-2(AQP2)与低肾素型高血压(LREH)发病机制的关系。方法选取LREH患者110例,分为1、2、3级高血压三个亚组,设置健康对照者40名,间接ELISA法检测尿液AQP2浓度,放射免疫法检测血浆血管加压素(AVP)浓度,监测平均动脉压(MAP),分析患者尿液AQP2浓度的变化及其与血浆AVP浓度、MAP之间的关系。结果LREH组不同血压级别三个亚组血浆AVP浓度、尿液AQP2浓度均高于健康对照组(t=2.99、t=3.01,P<0.05),并随血压级别升高而升高(P均<0.05);尿液AQP2浓度与血浆AVP浓度、MAP之间均呈正相关(r=0.862、r=0.694、r=0.797,P<0.05)。结论LREH患者肾脏集合管主细胞内AQP2穿梭、表达的增加。

Objective To discuss the effects of AQP2 in the pathogenesis of low renin essential hypertension group （LREH）. Methods According to the blood pressure grades, 110 patients with LREH were divided into three subgroup -the grade 1 hypertension subgroup,the grade 2 hypertension subgroup,the grade 3 hypertension subgroup. These patients were compared against 40 normal control subjects. In all subjects, the concentrations of u-AQP2 were detected by indirect enzyme linked immunosorbent assay （indirect ELISA）, the concentrations of plasma arginine vasopressin （p-AVP） were detected by radioimmunoassay （RIA）, and the mean arterial pressure （MAP） was measured. The relationship among u- AQP2, p-AVP and MAP was analysed. Results The concentrations of p-AVP and u-AQP2 in the patients in the three different subgroups with LREH were higher than that in control subjects （t=2.99,t=3.01,P〈0.05）,that increased along with the blood pressure grade （P〈0.05）. It had positive correlation among u-AQP2, p-AVP and MAP （r=0.862,r=0.694,r=0.797,P〈0.05）. Conclusion The shuttle and expression of AQP2 in the principle cell in the kidney colleting duct increased, that perhaps has relationship with the pathogenesis of water and salt retention in the patients with LREH .