E-选择素基因G98T多态性与原发性高血压发生率的相关性研究

Correlation between polymorphism of E-selectin G98T and blood pressure in patient with essential hypertension

目的：研究年龄在E-选择素G98T基因多态性对高血压影响中的作用。方法：高血压组326例，对照组216例。对2组进行血压测量，检测生化指标及一氧化氮（NO）、内皮素（ET）、血管紧张素I（AI）、血管紧张素H（AH）、醛固酮（ALD）。以聚合酶链反应-限制片段长度多态性（PCR-RFLP）方法进行E．选择素DNA多态性分析，电泳判断基因型并测序。结果：检测到TT、GG、GT3种基因型。在〈40岁组，携带T等位基因发生高血压的危险性为G的6．294倍[P=0．007；比值比（OR）=6．294；95％可信区间（CI）：1．361～29．11]。在40～60岁及〉60岁组，高血压组与对照组的等位基因频率差异无显著性（P：0．528和0．965）。〈40岁的HT组中TT—TG基因型诊室收缩压（CSBP）、诊室舒张压（CDBP）及平均动脉压（MAP）显著高于GG型（P分别为0．006、0．023和0．036）。在40～60岁组：TT-TG基因型的CSBP、MAP也显著高于GG基因型（P分别为0、031和0．042），而〉60岁组，两基因型间血压差异无显著性。结论：E-选择素G98T基因多态性与高血压显著相关，T等位基因携带者发生高血压风险大，血压值较高。

Objective： To investigate correlation between of E-selectin G98T polymorphism and blood pressure. Method： Genotypes of 326 essential hypertension patients[ male 173, female 153, age [ （47.12 ± 13.22） y, BMI （26.16± 3.33）kg/m^2] and 216 control individuals [male 60,female 156, age （40.04 ± 11.38）y, BMI （24.82 ± 3.04）kg/m^2] were divided into three age groups, 〈 40 y,40-60 y,and 〉 60 y. The polymorphism were typed by polymerase chain reaction restriction fragment length polymorphism （ PCR- RFLP）. The blood pressure and BG, TG, TC, LDL, HDL, UA, BUN, NO, ET, AI, All, ALD of each individuals were measured. Result： Three genotypes （ TY, GG,GT） were found. In 〈 40 y group, Risk of hypertension for allele T is 6.294 times of that for allele G（ P = 0.007 ;OR = 6.294;95 % CI：1.361-29.11 ）. In 40-60 y and 〉 60 y groups, no significantly difference in genotype were found either in HT patient or control. In HT group aged 〈 40 y, the CSBP,CDBP and MAP in TY-TG genotype are significantly higher than that in GG（ P = 0.006,0.023 and 0. 036）. Conclnsion： E-selectin G98T polymorphism is related with blood pressure. T allele has higher risk for hypertension and higer blood pressure. But this effect are con＇elated with age.