冷箭竹根际土壤中可培养细菌的多样性

Culturable bacterial diversity in rhizosphere of Bashania fangiana

为了解冷箭竹(Bashania fangiana)根际土壤中可培养细菌的多样性,2006年5月从四川卧龙自然保护区冷箭竹根际土壤中共分离出50株具不同菌落形态的细菌。16SrDNA序列分析结果表明:50株菌分属于10个属26个种。27株属于变形菌门γ亚群(Gammaproteobacteria)(42.3%)、9株属于厚壁菌门(Firmicutes)(26.9%)、4株属于放线菌门(Actinobacteria)(15.4%)、6株属于变形菌门β亚群(Betaproteobacteria)(7.7%)、3株属于变形菌门α亚群(Alphaproteobacteria)(3.9%),1株与土地杆菌属(Pedobacter)关系密切。假单胞菌属(Pseudomonas)和芽孢杆菌属(Bacillus)为优势菌属。2株菌为可能的新种或属。研究表明冷箭竹根际土壤中含有较为丰富的微生物多样性。

In order to understand the diversity of culturable bacteria, we collected Bashania fangiana rhizosphere soil in Wolong Nature Reserve in May 2006. Then we isolated and cultured 50 bacteria strains with different morphological character of colonies from the soil. Phylogenetic analysis based on the partial 16S rDNA sequences indicated that the 50 strains were clustered into ten genera： 27 strains of Gammaproteobacteria （42.3%）, nine strains of Firmicutes （26.9%）, four strains of Actinobacteria （15.4%）, six strains of Betaproteobacteria （7.7%）, three strains of Alphaproteobacteria （3.9%）, one strain closely related to the ge- nus Pedobacter. The dominant bacteria were Pseudomonas and Bacillus. Additionally, we found two potential novel species. The result shows that the bacterial population diversity is abundant in B. fangiana rhizosphere soil.