黄姜根茎组培快繁技术研究

Studies on Rhizome Culture and Rapid Propagation of Dioscorea Zingi berensis C.H.Wright

探索了黄姜离体培养快速繁殖的有效途径。结果表明:在附加6-BA的MS培养基上,外植体可诱导出大量的致密愈伤组织,而且致密愈伤组织在分化培养基中能分化产生大量圆球茎和幼苗;而在附加2,4-D的MS培养基中,外植体被诱导出疏松愈伤组织,在随后的分化培养基上只能产生数量有限的幼苗。带枝条的圆球茎在MS+NAA0.5 mg.L-1+IAA 0.5 mg.L-1生根培养基上的生根率达到88.2%;移栽成活率达85%。

The effective approach of rhizome culture and rapid propagation of Dioscorea Zingi berensis C. H. Wright was probed into in this paper. The results showed that： a great quantity of compacted callus were induced on the MS medium supplemented with 6-BA and a large amounts of corms and young seedlings were differentiated on the differentiation medium, but plenty of loosened callus on the MS medium supplemented with 2,4-D and limited young seedlings on the differentiation medium. The rooting rate of the corms with branches amounted to 88. 2% on the medium of MS＋NAA0.5 mg· L^-1 ＋IAA0.5 mg·L^-1 and the transplanting survival rate reached 85 %.