摘要
对L-组氨酸产生菌株发酵条件进行了优化,试验结果表明:种子培养基的最佳菌龄12h;菌种发酵周期60h。发酵培养基主要成分的质量浓度分别为葡萄糖100g/L,硫酸铵40g/L,玉米浆15g/L,硫酸镁2g/L。最佳发酵条件为300mL摇瓶的装液量30mL,接入2.25mL液体菌种,pH 7.4,转速130r/min。将其应用于产生菌株S6,其L-组氨酸最高产量达到了577mg/L,比原发酵条件的222mg/L提高了160%。
The fermentation conditions of L-Histidine production strain were optimized in this study. It was considered that the ideal strain age was 12 h; the cycle of the strain fermentation was 60 h. The optimal culture medium was： glucose 100 g/L, ammonium sulfate 40 g/L, cornsteep liquor 15 g/L, magnesium sulfate 2 g/L. The optimum fermentation conditions were： the volume of fermental liquid was 30 mL in the 300 mL bottle; the liquid seeds fluid was 2.25 mL, the pH of the medium was 7.4, the rotate speed of the culture machine was 130 r/min. Under the optimizing of the fermentation conditions, we get the product of L-Histidine was 577 mg/L by the strain of S6, the 1.6 times more than the former condition product of 222 mg/L.
出处
《中国酿造》
CAS
北大核心
2008年第1期30-32,共3页
China Brewing
基金
工业微生物湖北省重点实验室开放基金资助(湖工大科[2004]4号文)
关键词
L-His
发酵
残糖
菌龄
发酵周期
L-Histidine- fermentation
residue sugar
cell age
fermentation period
