微囊化转Maspin基因细胞对乳腺癌微血管密度及肺转移影响的实验研究

Influence of microencapsulated ovary cells modified with maspin gene on the microvessel density and lung metastasis of breast carcinoma

目的观察Maspin基因抑制肿瘤血管形成和肺转移的作用及微囊化转基因细胞体内治疗恶性肿瘤的可行性。方法将乳腺癌细胞Bcap37细胞注射到裸鼠背部皮下，制成荷瘤裸鼠动物模型，再用微囊化转Maspin基因的中国仓鼠卵巢上皮细胞（CHO细胞）进行干预治疗，1个月后，测量移植肿瘤的大小，免疫组化法检测肿瘤组织的血管内皮生长因子（VEGF）表达，并据此计算微血管密度（MVD），同时观察肺转移的情况。结果干预治疗1个月后，移植肿瘤的MVD明显降低[（26±9）与（60±16），P〈0.05]，肺转移率也明显减低（55％与15％，P〈0.05）。结论Maspin可明显抑制肿瘤血管形成和肺转移的发生，微囊化转基因细胞体内治疗恶性肿瘤是可行的。

Objective To observe the inhibition of maspin on the angiogenesis in tumor and lung metastasis of breast carcinoma and the feasibility of treatment of tumor by microencapsulated transgene cells in vivo. Methods Microencapsulated Chinese hamster ovarian epithelial ceils （CHO） modified with maspin gene, CHO/pcDNA3. 1/maspin cells, were prepared. Twenty BALB/C nude rats underwent subcutaneous injection of breast carcinoma cells of the line Bcap37 to establish tumor-loaded animal models and then randomly divided into 2 groups： maspin group, undergoing subcutaneous injection of CHO/ pcDNA3.1/maspin cells next to the transplanted tumor, and control group undergoing subcutaneous injection of microencapsulated CHO/pcDNA3. 1 cells. One month later, the rats were killed and the size and microvessel density （MVD） of the transplanted tumor and metastatic tumor in lung were observed. Results The MVD of the transplanted tumor of the maspin group was 26 ± 9, significantly lower than that of the control group （60 ± 16, P 〈 0.05 ）. The lung metastatic rate of the maspin group was 15% , significantly lower than that of the control group （55%, P 〈 0.05）. Condusion Maspin may inhibit the MVD in tumor and the occurrence of metastatic tumor in lung. It is feasible to use microencapsulated transgene cells as tumor-killer.