病毒载量检测有助于排除人免疫缺陷病毒抗体可疑结果中的非感染者

Viral load test conducive to excluding negative subjects from suspects in HIV antibody detections

目的研究病毒载量检测是否能够辅助排除人免疫缺陷病毒（human immunodeficiency virus，HIV）抗体可疑样本中的非感染者。方法选择在筛查实验中两种酶联试剂检测结果不一致（一阴一阳、样本S／CO比值〈6）、且确认实验检测结果为“HIV抗体可疑”的样本19份测定病毒载量。对这些个体的感染状况进行跟踪随访，观察6个月后抗体进展情况。结果19份筛查实验结果不一致的抗体可疑样本的病毒载量检测均为阴性，随访复查条带无进展，均排除感染HIV的可能。结论在抗体筛查实验和确认实验反应均很弱的条件下，病毒载量检测阴性有助于排除非HIV感染原因造成的抗体检测中的可疑结果。

Objective To study whether plasma viral load testing is helpful to exclude ones free from Human immunodeflciency virus （HIV）infections from suspects in HIV antibody detections. Methods 19 Specimens, which showed disconcordant results of the two HIV EIA testing （ S/CO 〈 6 ） and indeterminated results of Western blot （WB） test, were selected. Viral load of the specimens were detected. A six-month follow up survey in detecting HIV antibody was conducted in these subjects. Results None of these 19 cases was observed to be positive HIV viral loads and there was no any progress in WB bands development during the follow-up period. The possibility of HIV infection could be excluded. Conclusion When the specimens react with very low intensity in both EIA and WB, negative viral load result is conducive to exclude negative subjects from suspects in HIV antibody detections.