摘要
目的研究反义核酸技术封闭谷氨酰胺酶(GA)基因对裸鼠体内胃癌细胞凋亡作用的影响。方法将携带GA反义基因的质粒转染胃癌细胞SGC-7901,并将成功转染的胃癌细胞接种于裸鼠皮下,获得裸鼠胃癌移植瘤模型,通过TUNEL技术检测胃癌细胞凋亡的凋亡指数;RT-PCR技术检测移植瘤细胞内GA mRNA的含量。结果GA反义基因质粒载体成功转染胃癌细胞后,肿瘤生长速度减慢,癌细胞凋亡增加,肿瘤细胞内GA mRNA的含量减少。结论GA反义基因可抑制GA基因的表达,明显促进胃癌细胞凋亡。
Objective To study the effects of glutaminase (GA) gene blocked by antisense nucleotide on apoptosis of transplanted gastric carcinoma cells in nude mice. Methods The plasmid containing antisense sequence of GA gene was transfected into gastric carcinoma cells, then the cells were injected to endermic tissue of nude mice to create animal models of gastric carcinoma. Apoptosis of tumor cells was detected by terminal deoxynucleotidyl trans- ferase-mediated nick end labeling (TUNEL) method. The expression of GA mRNA in tumor tissue was measured by reverse transcription polymerase chain reaction (RT-PCR) technique. Results After the successful transfection of plasmid containing antisense sequence of GA gene into gastric carcinoma cells, the tumor's growth speed decreased, apoptosis of tumor cells increased, and the expression of GA mRNA also decreased. Conclusion The antisense gene of GA could inhibit the expression of GA gene and significantly increase the apoptosis of gastric carcinoma cells.
出处
《中国普外基础与临床杂志》
CAS
2008年第1期4-7,共4页
Chinese Journal of Bases and Clinics In General Surgery
基金
重庆市自然科学基金资助项目(编号:6828)~~
关键词
胃癌
反义核酸
谷氨酰胺酶
细胞凋亡
Gastric carcinoma Antisense nucleotide Glutaminase Cell apoptosis