摘要
目的探讨T细胞共刺激分子及其亚群在胃癌、大肠癌发生及预后中的作用。方法应用流式细胞术检测38例胃癌、42例大肠癌患者和21例健康人(对照组)外周血T细胞亚群及其共刺激分子CD28的表达。结果T细胞共刺激分子CD28(CD28+CD3+)表达:胃癌组为(25.80±10.56)%,大肠癌组为(28.95±9.29)%,均明显高于对照组的(0.82±0.98)%,P<0.01;总T细胞(CD3+)表达:胃癌组为(53.61±13.84)%,大肠癌组为(55.96±10.68)%,均明显低于对照组的(72.07±7.83)%,P<0.01;CD4+T细胞(CD4+CD3+)表达:胃癌组为(29.84±9.71)%,大肠癌组为(33.75±9.04)%,也均明显低于对照组的(38.79±5.08)%,P<0.01,P<0.05;细胞毒T细胞(CTL,CD8+CD28+CD3+)表达:胃癌组为(1.57±1.99)%,大肠癌组为(1.93±2.61)%,均明显高于对照组的(0.02±0.04)%,P<0.01;胃癌组CD8+抑制性T细胞(CD8+CD28-CD3+)和CD4/CD8比值明显低于对照组〔(16.06±6.94)%vs(20.56±6.54)%,P<0.05;(1.10±0.51)%vs(1.36±0.31)%,P<0.05〕;大肠癌组调节性T细胞(CD4+CD25+CD3+)明显高于对照组〔(19.74±6.89)%vs(13.72±3.08)%,P<0.01〕。胃癌组和大肠癌组患者手术前和手术后1周外周血T细胞亚群(除外胃癌组的CD3+细胞和CD28+CD3-细胞)的差异无统计学意义(P>0.05)。结论胃癌和大肠癌患者T细胞数量明显减少,T细胞共刺激分子CD28表达增高。胃癌患者CD4+T细胞显著减少;大肠癌患者调节性T细胞显著增加。
Objective To investigate the role of expression of T cell costimulatory molecule CD28 and variance of T cell subpopulations in the development and prognosis of gastric cancer and colorectal cancer. Methods The peripheral blood lymphocytes were tested for T cell subpopulations and T cell costimulatory molecule CD28 by flow cytometry in 38 patients with gastric cancer, 42 patients with colorectal cancer, and 21 healthy peoples as control group. Results Expressions of T cell costimulatory molecule CD28 in patients with gastric cancer and colorectal cancer were (25.80±10.56) % and (28.95±9.29) %, and significantly higher than that of control group [(0.82_±0.98) %, P〈0. 013. Expression percentage of total T cell (CD3+) in patients with gastric cancer and colorectal cancer were significantly lower than that of control group [ (53.61±13.84) % and (55.96 ± 10.68)% vs (72.07 ± 7.83) %, P〈0. 013. Expression percentage of CD4+ T cell (CD4+ CD3+ ) in patients with gastric cancer and colorectal cancer were significantly lower than that of control group [(29. 84 ± 9. 71) % and (33. 75 ± 9. 04) % vs (38.79±5.08) %; P〈0.01, P〈0. 053 ] Expression percentage of CTL cell (CD8+ CD28+ CD3+ ) in patients with gastric cancer and colorectal cancer were significantly higher than that of control group [(1. 57±1. 99)% and (1.93±2.61) % vs (0.02-1-0.04) % ; P〈0. 013 ] Expression percentage of CD8+ inhibitory T cell (CD8+ CD28- CD 3+) and CD4/CD8 ratio in patients with gastric cancer were significantly lower than that of control group[(16.06±6.94)% vs (20.56±6.54)%, P〈0.05; (1. 10±0.51)% vs (1.36±0.31)%, P〈0.05]; Expression of regulatory T cell (CD4+CD25+ CD3+) of patients with colorectal cancer was (19. 74± 6. 89)%, which was slgnificantly higher than that of control group [ ( 13.72 ± 3.08) %, P〈0.01]. No difference of expression was found in peripheral T cell subpopulations of postoperative patients with gastric cancer and colorectal cancer after one week (P〉0.05). Conclusion T cell number is [all, and T cell costimulatory molecule CD28 useless expression is increase in patients with gastric cancer and colorectal cancer. CD4+ T cell subpopulation is significantly de±creased in patients with gastric cancer. The regulatory T cell of patients with colorectal cancer is significantly increased.
出处
《中国普外基础与临床杂志》
CAS
2008年第1期51-55,共5页
Chinese Journal of Bases and Clinics In General Surgery
基金
福建省教育厅基金资助项目(编号:JB05245)~~
关键词
胃癌
大肠癌
共刺激分子
T细胞亚群
流式细胞术
Gastric cancer Colorectal cancer Costimulatory molecule T cell subpopulations Flow cytometry