T细胞共刺激分子及其亚群在胃癌和大肠癌组织中表达的意义

Expression of T Cell Costimulatory Molecule and Variance of T Cell Subpopulations in Patients with Gastric Cancer and Colorectal Cancer

目的探讨T细胞共刺激分子及其亚群在胃癌、大肠癌发生及预后中的作用。方法应用流式细胞术检测38例胃癌、42例大肠癌患者和21例健康人(对照组)外周血T细胞亚群及其共刺激分子CD28的表达。结果T细胞共刺激分子CD28(CD28+CD3+)表达:胃癌组为(25.80±10.56)%,大肠癌组为(28.95±9.29)%,均明显高于对照组的(0.82±0.98)%,P<0.01;总T细胞(CD3+)表达:胃癌组为(53.61±13.84)%,大肠癌组为(55.96±10.68)%,均明显低于对照组的(72.07±7.83)%,P<0.01;CD4+T细胞(CD4+CD3+)表达:胃癌组为(29.84±9.71)%,大肠癌组为(33.75±9.04)%,也均明显低于对照组的(38.79±5.08)%,P<0.01,P<0.05;细胞毒T细胞(CTL,CD8+CD28+CD3+)表达:胃癌组为(1.57±1.99)%,大肠癌组为(1.93±2.61)%,均明显高于对照组的(0.02±0.04)%,P<0.01;胃癌组CD8+抑制性T细胞(CD8+CD28-CD3+)和CD4/CD8比值明显低于对照组〔(16.06±6.94)%vs(20.56±6.54)%,P<0.05;(1.10±0.51)%vs(1.36±0.31)%,P<0.05〕;大肠癌组调节性T细胞(CD4+CD25+CD3+)明显高于对照组〔(19.74±6.89)%vs(13.72±3.08)%,P<0.01〕。胃癌组和大肠癌组患者手术前和手术后1周外周血T细胞亚群(除外胃癌组的CD3+细胞和CD28+CD3-细胞)的差异无统计学意义(P>0.05)。结论胃癌和大肠癌患者T细胞数量明显减少,T细胞共刺激分子CD28表达增高。胃癌患者CD4+T细胞显著减少;大肠癌患者调节性T细胞显著增加。

Objective To investigate the role of expression of T cell costimulatory molecule CD28 and variance of T cell subpopulations in the development and prognosis of gastric cancer and colorectal cancer. Methods The peripheral blood lymphocytes were tested for T cell subpopulations and T cell costimulatory molecule CD28 by flow cytometry in 38 patients with gastric cancer, 42 patients with colorectal cancer, and 21 healthy peoples as control group. Results Expressions of T cell costimulatory molecule CD28 in patients with gastric cancer and colorectal cancer were （25.80±10.56） % and （28.95±9.29） %, and significantly higher than that of control group [（0.82_±0.98） %, P〈0. 013. Expression percentage of total T cell （CD3＋） in patients with gastric cancer and colorectal cancer were significantly lower than that of control group [ （53.61±13.84） % and （55.96 ± 10.68）% vs （72.07 ± 7.83） %, P〈0. 013. Expression percentage of CD4＋ T cell （CD4＋ CD3＋ ） in patients with gastric cancer and colorectal cancer were significantly lower than that of control group [（29. 84 ± 9. 71） % and （33. 75 ± 9. 04） % vs （38.79±5.08） %; P〈0.01, P〈0. 053 ] Expression percentage of CTL cell （CD8＋ CD28＋ CD3＋ ） in patients with gastric cancer and colorectal cancer were significantly higher than that of control group [（1. 57±1. 99）% and （1.93±2.61） % vs （0.02-1-0.04） % ; P〈0. 013 ] Expression percentage of CD8＋ inhibitory T cell （CD8＋ CD28- CD 3＋） and CD4/CD8 ratio in patients with gastric cancer were significantly lower than that of control group[（16.06±6.94）% vs （20.56±6.54）%, P〈0.05; （1. 10±0.51）% vs （1.36±0.31）%, P〈0.05]; Expression of regulatory T cell （CD4＋CD25＋ CD3＋） of patients with colorectal cancer was （19. 74± 6. 89）%, which was slgnificantly higher than that of control group [ （ 13.72 ± 3.08） %, P〈0.01]. No difference of expression was found in peripheral T cell subpopulations of postoperative patients with gastric cancer and colorectal cancer after one week （P〉0.05）. Conclusion T cell number is [all, and T cell costimulatory molecule CD28 useless expression is increase in patients with gastric cancer and colorectal cancer. CD4＋ T cell subpopulation is significantly de±creased in patients with gastric cancer. The regulatory T cell of patients with colorectal cancer is significantly increased.