NK细胞抑制CD34^+早期急性髓系白血病细胞克隆形成

Cytotoxicity of allogenetic NK cells against CD34^+ early acute myelogenous leukemia cells

目的探讨同种异体NK细胞对CD34+早期急性髓系白血病细胞的克隆抑制效应。方法免疫磁珠法分离5例健康个体NK细胞,甲基纤维素克隆形成实验检测NK细胞对KG1a细胞杀伤后的克隆形成,同时与NK杀伤敏感细胞株K562比较。结果急性髓系白血病细胞株KG1a中CD34抗原表达率为(98.0±1.1)%,分选后的NK细胞(CD3-CD16+CD56+细胞)纯度为(93.2±3.7)%。不同效靶比时NK细胞对KG1a细胞均能抑制其克隆形成。随着效靶比的增高,其克隆抑制率随之增高(P<0.05)。同一效靶比时,NK细胞对KG1a的克隆抑制率低于K562,差异有显著性(P<0.05)。结论同种异体NK细胞对CD34+早期急性髓系白血病细胞的克隆形成具有一定的抑制作用。

Objective To study the in vitro clone inhibition rate of allogenetic NK ceils on human CD34^＋ early acute myelogenous leukemia cells. Methods The expression of CD34 antigen on acute myelogenous leukemia cell line - KG1a cells was detected by FACS. Clone inhibition rate of NK cells isolated from 5 healthy persons with MACS was detected by calculation of methylcellulose at different effect -to -target （E：T） ratios compared with K562 which is sensitive to NK cells. Results The expression rate of CD34 antigen on KG1a cells was （98.0 ± 1.1 ） % and purify of isolated NK cells （ CD3^- CD16^＋ CD56^＋ cells） was （93.2 ± 3.7 ） % detected by FACS. There was in vitro clone inhibition of allogenetic NK cells against KG1 a cells at different E ：T ratios. With increasing E： T ratios, clone inhibition rate of allogenetic NK cells against KGla cells increased. With same E： T ratios, clone inhibition rate against KGla cells was lower than that against K562 cells （ P 〈 0.05 ）. Conclusion Allogenetic NK cells displayed clone inhibition against CD34^＋ early acute myelogenous leukemia cells.