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rIL-18对肺炎链球菌肺炎小鼠Th1/Th2免疫应答的影响 被引量：5

Therapeutic effects of recombinant interleukin-18 on Th1/Th2 immune responses in mice with pneumococcal pneumonia
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摘要 目的研究重组白细胞介素18(rIL-18)对肺炎链球菌肺炎小鼠Th1/Th2免疫应答的影响。方法鼻腔接种肺炎链球菌建立小鼠肺炎链球菌肺炎模型,将Balb/c小鼠24只随机分为3组,分别为对照组,肺炎组和肺炎rIL-18干预组(n=8),RT-PCR法检测各组小鼠肺组织中IFN-γ、IL-4mRNA的表达,同时支气管肺泡灌洗液(BALB)进行活菌计数,有核细胞分类计数。结果①肺炎rIL-18干预组BALF中性粒细胞和巨噬细胞计数显著高于肺炎组和对照组(P<0.001);②肺炎rIL-18干预组BALF活菌计数显著低于肺炎组(P<0.001);③肺炎rIL-18干预组肺组织IFN-γmRNA表达上调而IL-4mRNA表达下调(P<0.001)。结论在小鼠肺炎链球菌肺炎早期给予rIL-18可诱导IFN-γ的合成,促进Th1免疫应答,使Th1/Th2免疫平衡向Th1免疫偏移、促进宿主对肺炎链球菌的防御。 Objective To investigate the effects of recombinant interleukin-18 （rIL-18） on Thl/Th2 immune responses in mice with pneumococcal pneumonia. Methods Total of 24 Balb/c mice randomized into normal control, pneumococcal pneumonia, and pneumococcal pneumonia plus rIL-18 groups （n = 8）, were treated with normal sodium or rIL-18 respectively. The expressions of IFN-γ and IL-4 mRNA in lung were detected with RT-PCR. The numbers of viable bacteria and nucleated cell in bronchoalveolar lavage fluid （BALF） were counted. Results The number of neutrophil and macrophage in BALF of rIL-18 group was larger than that normal control group and pneumococcal pneumonia group （ P 〈 0.001 ）. The number of viable bacteria in BALF of rlL-18 group was smaller than that of the other two groups （ P 〈 0. 001）. rlL-18 could up-regulate the expression of IFN-γ mRNA and down-regulate the expression of IL-4mRNA in lung （ P 〈 0. 001 ）. Conclusion At the initial stage of pneumococcal pneumonia, rlL-18 could induce IFN-γ production, promote the development of Thl cell, and modulate Thl and Th2 cytokine balance, suggesting that rIL-18 could enhance the host defense against Streptococcus pneumoniae.
作者 熊盛道 徐国鹏 熊维宁 舒俊华 倪望 陈仕新
机构地区 华中科技大学同济医学院附属同济医院呼吸内科
出处 《免疫学杂志》 CAS CSCD 北大核心 2008年第1期69-72,共4页 Immunological Journal
关键词 白细胞介素18 肺炎链球菌肺炎 免疫应答 Interleukin- 18 Pneumococcal pneumonia Immune response
分类号 R392 [医药卫生—免疫学]
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参考文献13

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二级参考文献21

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共引文献28

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免疫学杂志
2008年 第1期

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