肺癌组织的HLA-Ⅰ类分子表达与临床病理因素关系

Expression of human leukocyte antigen-Ⅰ in lung cancer

目的研究肺癌组织的人类白细胞抗原I类分子HLA-A,B,C,G的表达情况以及临床病理因素关系。方法抗HLA-A,B,C单克隆抗体(mAb)W6/32,抗HLA-G(mAb)GG11,以免疫组化S-P法检测49例肺癌中的HLA-A,B,C,G抗原表达情况,同时收集肺癌患者临床特征。结果小细胞肺癌、鳞癌和腺癌的HLA-A,B,C抗原表达有不同程度的下降或缺失,小细胞肺癌HLA-A,B,C抗原下降和缺失率最高(P<0.05);而不同病理分期、分级的肺癌HLA-A,B,C抗原表达并无差异(P>0.05)。HLA-G在49例原发肺癌癌细胞中均无阳性表达,但4例肿瘤组织旁的部分免疫细胞上有阳性表达。结论肺癌HLA-A,B,C抗原表达下降与肺癌组织类型相关,而与临床病理因素无关;HLA-G在肺癌细胞无表达,而部分免疫细胞表达HLA-G。

Objective To analyze the association of HLA-A, B, C, G expression status with clinical and pathologic characteristics of lung cancer. Methods Forty-nine tumor samples contained sufficient material were gathered for immunohistochemical analysis. HLA-A, B, C and HLA-G antigens were assayed using monoclonal antibody （W6/32 and GGll） respectively. A database of clinical and pathologic characteristics was established. Results A loss of HIA-A, B, C was obviously observed in cancer tissues, especially in small cell cancers （ P 〈 0.05）. HIA G was existed in cancer tissues, but loss in some tumor infiltrating immune cell. No significant correlations were observed among HIA-A, B, C expression and tumor size, nodal involvement （ P 〉 O. 05）. Conclusion Different histological types of lung cancer show a different loss of HIA-A,B,C, but loss of HIA-G only in some tumor infiltrating immune cell. HIA-A, B, C expression is not related with clinical and pathologic characteristics. Lung cancer could escape from the host immune system through HIA class I-induced immune tolerance.