反复CVB_(3m)感染小鼠病理学及免疫学研究

Pathology and immunology studies of repetitive CVB3m infection of mouse

目的研究反复柯萨奇病毒B3m(CVB3m)感染对小鼠的影响,建立可行的病毒性心肌病动物模型。方法115只3～4周龄雄性Balb/c小鼠随机分为增量感染组,等量感染组,单次感染组和正常对照组,于首次感染病毒后第104天处死小鼠,应用阻抗微分法测定心输出量,病理及组织化学技术分析心肌病理损伤和胶原系统改变,计算胶原容积分数。ELISA法测定血清sIL-2R含量。结果增量感染组小鼠死亡率持续增高,心脏重量增加,心功能下降,心肌胶原容积分数和血清sIL-2R含量高于其它各组(P<0.05),病理学特征为基质胶原明显增生重建。结论反复增量病毒感染诱发小鼠心脏重构和免疫系统功能异常可做为病毒性心肌病模型进一步研究。

Objective To study the influence of repetitive CVB3m infection on mice and establish viral cardiomyopathy model. nathods Total of 115 male Balb/c mice, 3-4 week old, were randomly divided into increasing dose group, same dose group, single infection group, and normal control group. The mice were sacrificed on 104 days post first infection. Impedance differentiation assay was used to calculate cardiac output （C. O）. The changes of myocardial pathology and collagen were detected by pathology and histochemistry techniques. The collagen volume fraction was counted. The content of sIL-2R in blood serum was detected by ELISA. Results In increasing dose group, the death rate and the heart weight were increased but the cardiac function were decreased. The collagen volume fraction and the sIL-2R content in blood of serum increasing dose group were higher than that of the other groups （ P 〈 0.05 ）. The pathologic feature was the significant proliferation and reconstruction of the extracellular matrix collagen. Conclusion The repetitive and increasing CVB3m infection induce cardiac remodeling and the immune system disorder in mouse. The established model can be used as viral cardiomyopathy model to future investigation.