食品中产单核细胞李斯特菌PCR检测方法的建立

Establishment of PCR detection Method for Listeria monocytogenes in Food

根据产单核细胞李斯特菌hlyA基因设计引物,进行PCR扩增,检测该方法的特异性和灵敏度。人工污染样品经Half-fraser和Fraser增菌后进行PCR检测。结果表明,产单核细胞李斯特菌扩增出234bp的条带,对照菌未扩增出目的条带。该方法的灵敏度为104cfu/mL。人工污染样品的检出限为8cfu/25g,说明PCR方法检测食品中产单核细胞李斯特菌具有快速、特异、敏感等特点,具有较高的实用价值。

A PCR was established for the detection of Listeria monoeytogenes （LM） in food with a pair of primers specific to the LM hly A gene. The sensitivity and specificity of this method were detected.. The procedure was validated by detecting Listeria monocytogenes in artificially contaminated food and PCR analysis was taken following Half-fraser and fraser enrichment steps. The specific 234 bp fragment was amplified in LM while none was amplified in all other species. The specificity is 104 cfu /mL.As few as 8 cfu /25 g can be detected in artificially contaminated food. The PCR procedure has proved to be a rapid sensitive and specific method suitable for detecting Listeria monocytogenes in food.